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rabbit anti mouse p smad2 monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti mouse p smad2 monoclonal antibody
    (A) Western blot of the effect of recombinant LAP on α-SMA, <t>Smad2</t> and p-Smad2 expression in H9C2 cells induced by TGF-β1; +: TGF-β1+LAP group; + 1 : LAP pre-protection group. (B)–(D) Semi-quantitative analysis of α-SMA, Smad2 and p-Smad2 expression presented as the relative ratio to GAPDH. The expression of α-SMA ( P < 0.0001) and p-Smad2 ( P = 0.0003) in the TGF-β1 model group was significantly increased compared with the control group. Compared with the TGF-β1 model group, both TGF-β1+LAP group and LAP pre-protection group significantly reduced the increase of α-SMA (TGF-β1 vs TGF-β1+LAP, P = 0.0409; TGF-β1 vs LAP Pre-protection, P = 0.008) and P-Smad2 (TGF-β1 vs TGF-β1+LAP, P = 0.0285; TGF-β1 vs LAP Pre-protection, P = 0.0044) levels. NC: control group; LAP: 60 μg/mL LAP group; TGF-β1: 10 ng/mL TGF-β1 group; TGF-β1+LAP: Add 10 ng/mL TGF-β1 first and then 60 μg/mL LAP treatment group; LAP pre-protection group: 10ng/mL TGF-β1 and 60μg/mL LAP at the same time. n = 3, *** P < 0.001 vs NC group; # P < 0.05 vs TGF-β1 group; ## P < 0.01 vs TGF-β1 group.
    Rabbit Anti Mouse P Smad2 Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse p smad2 monoclonal antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 195 article reviews
    rabbit anti mouse p smad2 monoclonal antibody - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Prokaryotic expression, purification and evaluation of anti-cardiac fibrosis activity of recombinant TGF-β latency associated peptide"

    Article Title: Prokaryotic expression, purification and evaluation of anti-cardiac fibrosis activity of recombinant TGF-β latency associated peptide

    Journal: PeerJ

    doi: 10.7717/peerj.12797

    (A) Western blot of the effect of recombinant LAP on α-SMA, Smad2 and p-Smad2 expression in H9C2 cells induced by TGF-β1; +: TGF-β1+LAP group; + 1 : LAP pre-protection group. (B)–(D) Semi-quantitative analysis of α-SMA, Smad2 and p-Smad2 expression presented as the relative ratio to GAPDH. The expression of α-SMA ( P < 0.0001) and p-Smad2 ( P = 0.0003) in the TGF-β1 model group was significantly increased compared with the control group. Compared with the TGF-β1 model group, both TGF-β1+LAP group and LAP pre-protection group significantly reduced the increase of α-SMA (TGF-β1 vs TGF-β1+LAP, P = 0.0409; TGF-β1 vs LAP Pre-protection, P = 0.008) and P-Smad2 (TGF-β1 vs TGF-β1+LAP, P = 0.0285; TGF-β1 vs LAP Pre-protection, P = 0.0044) levels. NC: control group; LAP: 60 μg/mL LAP group; TGF-β1: 10 ng/mL TGF-β1 group; TGF-β1+LAP: Add 10 ng/mL TGF-β1 first and then 60 μg/mL LAP treatment group; LAP pre-protection group: 10ng/mL TGF-β1 and 60μg/mL LAP at the same time. n = 3, *** P < 0.001 vs NC group; # P < 0.05 vs TGF-β1 group; ## P < 0.01 vs TGF-β1 group.
    Figure Legend Snippet: (A) Western blot of the effect of recombinant LAP on α-SMA, Smad2 and p-Smad2 expression in H9C2 cells induced by TGF-β1; +: TGF-β1+LAP group; + 1 : LAP pre-protection group. (B)–(D) Semi-quantitative analysis of α-SMA, Smad2 and p-Smad2 expression presented as the relative ratio to GAPDH. The expression of α-SMA ( P < 0.0001) and p-Smad2 ( P = 0.0003) in the TGF-β1 model group was significantly increased compared with the control group. Compared with the TGF-β1 model group, both TGF-β1+LAP group and LAP pre-protection group significantly reduced the increase of α-SMA (TGF-β1 vs TGF-β1+LAP, P = 0.0409; TGF-β1 vs LAP Pre-protection, P = 0.008) and P-Smad2 (TGF-β1 vs TGF-β1+LAP, P = 0.0285; TGF-β1 vs LAP Pre-protection, P = 0.0044) levels. NC: control group; LAP: 60 μg/mL LAP group; TGF-β1: 10 ng/mL TGF-β1 group; TGF-β1+LAP: Add 10 ng/mL TGF-β1 first and then 60 μg/mL LAP treatment group; LAP pre-protection group: 10ng/mL TGF-β1 and 60μg/mL LAP at the same time. n = 3, *** P < 0.001 vs NC group; # P < 0.05 vs TGF-β1 group; ## P < 0.01 vs TGF-β1 group.

    Techniques Used: Western Blot, Recombinant, Expressing, Control



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    rabbit anti mouse p smad2 monoclonal antibody  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc rabbit anti mouse p smad2 monoclonal antibody
    (A) Western blot of the effect of recombinant LAP on α-SMA, <t>Smad2</t> and p-Smad2 expression in H9C2 cells induced by TGF-β1; +: TGF-β1+LAP group; + 1 : LAP pre-protection group. (B)–(D) Semi-quantitative analysis of α-SMA, Smad2 and p-Smad2 expression presented as the relative ratio to GAPDH. The expression of α-SMA ( P < 0.0001) and p-Smad2 ( P = 0.0003) in the TGF-β1 model group was significantly increased compared with the control group. Compared with the TGF-β1 model group, both TGF-β1+LAP group and LAP pre-protection group significantly reduced the increase of α-SMA (TGF-β1 vs TGF-β1+LAP, P = 0.0409; TGF-β1 vs LAP Pre-protection, P = 0.008) and P-Smad2 (TGF-β1 vs TGF-β1+LAP, P = 0.0285; TGF-β1 vs LAP Pre-protection, P = 0.0044) levels. NC: control group; LAP: 60 μg/mL LAP group; TGF-β1: 10 ng/mL TGF-β1 group; TGF-β1+LAP: Add 10 ng/mL TGF-β1 first and then 60 μg/mL LAP treatment group; LAP pre-protection group: 10ng/mL TGF-β1 and 60μg/mL LAP at the same time. n = 3, *** P < 0.001 vs NC group; # P < 0.05 vs TGF-β1 group; ## P < 0.01 vs TGF-β1 group.
    Rabbit Anti Mouse P Smad2 Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit monoclonal anti mouse antibody p smad2 3  (Cell Signaling Technology Inc)
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    (A) Western blot of the effect of recombinant LAP on α-SMA, <t>Smad2</t> and p-Smad2 expression in H9C2 cells induced by TGF-β1; +: TGF-β1+LAP group; + 1 : LAP pre-protection group. (B)–(D) Semi-quantitative analysis of α-SMA, Smad2 and p-Smad2 expression presented as the relative ratio to GAPDH. The expression of α-SMA ( P < 0.0001) and p-Smad2 ( P = 0.0003) in the TGF-β1 model group was significantly increased compared with the control group. Compared with the TGF-β1 model group, both TGF-β1+LAP group and LAP pre-protection group significantly reduced the increase of α-SMA (TGF-β1 vs TGF-β1+LAP, P = 0.0409; TGF-β1 vs LAP Pre-protection, P = 0.008) and P-Smad2 (TGF-β1 vs TGF-β1+LAP, P = 0.0285; TGF-β1 vs LAP Pre-protection, P = 0.0044) levels. NC: control group; LAP: 60 μg/mL LAP group; TGF-β1: 10 ng/mL TGF-β1 group; TGF-β1+LAP: Add 10 ng/mL TGF-β1 first and then 60 μg/mL LAP treatment group; LAP pre-protection group: 10ng/mL TGF-β1 and 60μg/mL LAP at the same time. n = 3, *** P < 0.001 vs NC group; # P < 0.05 vs TGF-β1 group; ## P < 0.01 vs TGF-β1 group.
    Rabbit Monoclonal Anti Mouse Antibody P Smad2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Western blot of the effect of recombinant LAP on α-SMA, Smad2 and p-Smad2 expression in H9C2 cells induced by TGF-β1; +: TGF-β1+LAP group; + 1 : LAP pre-protection group. (B)–(D) Semi-quantitative analysis of α-SMA, Smad2 and p-Smad2 expression presented as the relative ratio to GAPDH. The expression of α-SMA ( P < 0.0001) and p-Smad2 ( P = 0.0003) in the TGF-β1 model group was significantly increased compared with the control group. Compared with the TGF-β1 model group, both TGF-β1+LAP group and LAP pre-protection group significantly reduced the increase of α-SMA (TGF-β1 vs TGF-β1+LAP, P = 0.0409; TGF-β1 vs LAP Pre-protection, P = 0.008) and P-Smad2 (TGF-β1 vs TGF-β1+LAP, P = 0.0285; TGF-β1 vs LAP Pre-protection, P = 0.0044) levels. NC: control group; LAP: 60 μg/mL LAP group; TGF-β1: 10 ng/mL TGF-β1 group; TGF-β1+LAP: Add 10 ng/mL TGF-β1 first and then 60 μg/mL LAP treatment group; LAP pre-protection group: 10ng/mL TGF-β1 and 60μg/mL LAP at the same time. n = 3, *** P < 0.001 vs NC group; # P < 0.05 vs TGF-β1 group; ## P < 0.01 vs TGF-β1 group.

    Journal: PeerJ

    Article Title: Prokaryotic expression, purification and evaluation of anti-cardiac fibrosis activity of recombinant TGF-β latency associated peptide

    doi: 10.7717/peerj.12797

    Figure Lengend Snippet: (A) Western blot of the effect of recombinant LAP on α-SMA, Smad2 and p-Smad2 expression in H9C2 cells induced by TGF-β1; +: TGF-β1+LAP group; + 1 : LAP pre-protection group. (B)–(D) Semi-quantitative analysis of α-SMA, Smad2 and p-Smad2 expression presented as the relative ratio to GAPDH. The expression of α-SMA ( P < 0.0001) and p-Smad2 ( P = 0.0003) in the TGF-β1 model group was significantly increased compared with the control group. Compared with the TGF-β1 model group, both TGF-β1+LAP group and LAP pre-protection group significantly reduced the increase of α-SMA (TGF-β1 vs TGF-β1+LAP, P = 0.0409; TGF-β1 vs LAP Pre-protection, P = 0.008) and P-Smad2 (TGF-β1 vs TGF-β1+LAP, P = 0.0285; TGF-β1 vs LAP Pre-protection, P = 0.0044) levels. NC: control group; LAP: 60 μg/mL LAP group; TGF-β1: 10 ng/mL TGF-β1 group; TGF-β1+LAP: Add 10 ng/mL TGF-β1 first and then 60 μg/mL LAP treatment group; LAP pre-protection group: 10ng/mL TGF-β1 and 60μg/mL LAP at the same time. n = 3, *** P < 0.001 vs NC group; # P < 0.05 vs TGF-β1 group; ## P < 0.01 vs TGF-β1 group.

    Article Snippet: Then, a rabbit anti-mouse collagen I monoclonal antibody (Affinity Biosciences, Cincinnati, OH, USA, 1:1,000 dilution), a rabbit anti-mouse α-SMA monoclonal antibody (Affinity Biosciences, Cincinnati, OH, USA, 1:1,000 dilution), a rabbit anti-mouse FN monoclonal antibody (Affinity Biosciences, Cincinnati, OH, USA, 1:1,000 dilution), a rabbit anti-mouse Smad2 monoclonal antibody (CST, USA, 1:1,000 dilution) and a rabbit anti-mouse p-Smad2 monoclonal antibody (CST, USA, 1:1,000 dilution) were added separately overnight at 4 °C.

    Techniques: Western Blot, Recombinant, Expressing, Control